[Effect of chrysin on AGS human gastric cancer cell line]

Chrysin is a natural and active biological component which is extracted from plants, honey and propolis. Chrysin has anti-inflammatory, anticancer and antioxidant propertis. This study was done to evaluate the effect of chrysin on AGS human gastric cancer cell line. In this descriptive - analytic study, chrysin was dissolved in dimethyl sulfoxide [DMSO] and the cytotoxic effects of concentrations of 10, 15, 20, 30, 40 ,50, 60, 70, 80, and 100 µM/ml of chrysin on AGS cells was evaluated. Viability of the cells was determined with MTT assay after 24, 48 and 72 hours and compared to controls. Chrysin inhibited the growth and proliferation of human gastric cancer AGS cell line. The antiproliferative effect of chrysin was dose and time dependent. The IC50 values were determined for 60, 30 and 20 µM, in incubation time of 24, 48 and 72 hour, respectively [P<0.05]. Chrysin proved to have antiproliferative activity on human gastric cancer cells in culture medium

[ accuracy of cone-beam computerized tomography linear measurements in human dry mandible]

The aim of this study was to evaluate the accuracy of Cone-beam Computerized Tomography [CBCT] linear measurements in dry mandibles by comparing them with direct measurements. Two human dry mandibles were used in this study. Thirteen sites were selected on each mandible. Specimens were scanned by CBCT and linear measurements were made using Planmeca Romexis 2.3.0.R software. Mandibles were cut into sections at marked locations and the same distances were measured by a digital caliper. Differences between two methods were assessed using paired t- test. The mean differences for bone height, bone width and the crest-canal distance between CBCT and digital caliper measurements were -0.47 +/- 1 mm, -0.14 +/- 0.78 mm and 0.00 +/- 0.76 mm respectively. The differences were only, significant for bone height measurements [P<0.05]. There was a high carreltion between the result of CBCT and gold standard. The results of this in-vitro study on mandible indicate that, although there is a statistically significant difference for bone height measurements between the two methods, the difference is not significant in Clinical applications. Thus, CBCT was shown to be a useful technique for preoperative dental implant planning

Effect of aqueous and alcoholic extracts of roots of Ferula gummosa Boiss. on the growth of Pseudomonas aeruginosa

Ferula gummosa Boiss. [Barije.] contain medical and antimicrobial properties. This study was done to determine the effect of aqueous and alcoholic extracts of roots of Ferula gummosa Boiss. on the growth of Pseudomonas aeruginosa. In this laboratory study, the plant was dried in dark place and aqueous, alcoholic extracts of Barije's root, powder were prepared using Soxhlet method. The efficacy of 0.1 dilution of different values of extracts of Ferula gummosa Boiss. on the strain of Pseudomonas aeruginosa [PTCC 1430] were evaluated by disk diffusion, Agar-well diffusion, minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC] methods. Pseudomonas aeruginosa was completely resistant to the aqueous extract, and the MIC for the methanol and ethanol extracts was 1.25x10[4] microg/ml and 6.25x10[3] microg/ml, respectively. Methanolic and ethanolic extracts of Ferula gummosa Boiss. have antimicrobial activity against Pseudomonas aeruginosa in in-vitro model

[Protective effect of delayed ischemic postconditioning on embolic stroke complications in female rat]

Background and Aims: Ischemic postconditioning, conducted by a series of brief occlusion and release of the bilateral common carotid arteries, has neuroprotective properties in permanent or transient models of middle cerebral artery [MCA] occlusion but its delayed neuroprotective effects in the embolic model of stroke, especially in female rat, have not yet been reported and were investigated in the current study

Methods: In this experimental study, 24 female Wistar rats [200 to 250 g] were divided into three groups of sham, stroke and postconditioning. All animals were similar in reproductive cycle, and after that, stroke was induced by clot injection into the right middle cerebral artery. For postconditioning, common carotid arteries [CCA] were occluded for 30 seconds and reopened for 30 seconds, for 5 cycles. The postconditioning was induced at 6.5 hours after the stroke. Infarction volume, brain edema and neurological deficits were measured two days later

Results: Postconditioning at 6.5 hours after stroke decreased infarction volume [P< 0.001], brain edema [P<0.050] and norologic deficit [P<0.050] significantly compared to the stroke group

Conclusion: Late postconditioning improved ischemic injury, brain edema and neurological functions after the embolic model of stroke in female rat

Assessment of stress resistance of industrial strains of Saccharomyces cerevisiae for designing selection media

The two main methods for obtaining microbial strains with specific characteristics for application in the industry are isolation from natural sources and using random mutagenesis. Characterization of all isolated strains is very time-consuming and expensive. In this study the tolerance of some strains of Saccharomyces cerevisiae to different stresses was measured and the association between these stresses and tolerance to osmotic pressure and production of intracellular trehalose determined, aiming at applying the results to designing selection media. The viability [percent cell survival] of different strains of Saccharomyces cerevisiae was assessed by exposure to a 3M Nacl solution, a 40% sorbitol solution, a freezing shock at -20°C, and a heat shock at 52°C. In addition, the intracellular accumulation of trehalose was determined by the antrone reagent. The associations between these factors and resistance of Saccharomyces cerevisiae strains were then determined using statistical tests. Strong correlations were observed between resistance to NaCl- and sorbitol-introduced stress and strains of Saccharomyces cerevisiae [p<0.01]. There was also a strong association between intracellular trehalose accumulation and resistance to heat shock [p<0.01]. While sugars can not select osmotolerant cells, Nacl is a very strong selector for more specific isolation of more resistant cells in a suspension. Similarly, heat shock stress is very efficient in selecting cells with a higher intracellular trehalose accumulation in a suspension

role of rheumatoid factor and anti-cyclic citrullinated peptide antibody in diagnosis of rheumatoid arthritis

Rheumatoid Factor [RF] occurs in 70- 90% of patients with established rheumatoid arthritis [RA]. Anti-cyclic Citrullinated peptide antibody [Anti-CCP] detection may be used to confirm the diagnosis of RA in patients with uncharacterized chronic inflammatory arthritis. This study is an attempt to evaluate the role of RF and anti-CCP in the diagnosis and prognosis of rheumatoid arthritis. Blood samples were obtained from 55 patients with established RA and 55 healthy controls. Anti-CCP and RF were measured by ELISA and nephelometry. The patient's demographics, disease duration, DMARDs usage, ESR and radiographic abnormalities were recorded. Fifty five RA patients and 55 healthy age and sex matched controls were enrolled. Odd ratio [OR] was 87.42 [C 1=11.2-680.1] for RF and 27.48 [6/08-124/09] for Anti- CCP. Five patients had used more than 3 DMARDs; all of them were RF positive and 4 were also anti-CCP positive. Eleven patients had erosions in their radiographs; 10 of them were seropositive for RF and 9 positive for anti-CCP. Our study suggests that despite lack of specificity, RF continues to be a central part of the definition of RA due to favorable sensitivity profile and the combined use of RF. Also, anti-CCP is a more powerful diag-nostic and prognostic tool

[Diagnostic values of conventional radiography and digital subtraction radiography in detection of periapical bone lesions [an invitro study]]

The detection of the teeth periapical lesions is associated with some problems clinically. As this diagnosis is based on the observation of radiolucent regions in the periapical area, the radiographic assessmcnts of the lesions are important. The aim of the present study was to evaluate the diagnostic values of conventional and digital subtraction radiography to improve the diagnostic ability of periapical lesions. Artificial bone lesions in the periapical region of 15 teeth were created. Conventional and digital radiography were taken of all specimens in four stages: preoperative and after lesion creation in cancellous bone by using No.2, No.6 and No.10 slow speed steel burs. Digital radiography images were exposcd wherc as the distance between x-ray source and CCD was 24cm and position of tube, tecth and receptor was fixed. The data was analyzed by kappa statistics. Diagnostic sensitivity in detection of lesion by conventional radiography and digital subtraction radiography by using bur No. 2 were 34.5%, 71.4%, No. 6 were 54.7%, 95.2% and bur No.10 slow speed steel bur were 92.9%, 100% respectively. The digital subtraction radiography in comparison with conventional radiography had a higher diagnostic value in detection of periapical bone lesions

Anticardiolipin antibodies in patients with ischemic events

To determine the presence of anticardiolipin [aCL] antibodies in patients with ischemic events, we designed a case-control study. We studied 33 patients with unstable angina, 33 male patients with myocardial infarction and 34 control subjects with no evidence of ischemic heart disease. Plasma samples were assessed for IgG anticardiolipin antibodies by enzyme-linked immunosorbent assay [ELISA]. The levels of aCL were [mean +/- SD of optical density multiplied by 1000]: 624 +/- 319, 486 +/- 318, and 239 +/- 202 for patients with unstable angina, myocardial infarction and controls, respectively [F=15.74 and p=0.0000]. High aCL levels were found more often in patients with acute ischemic events